The present study deals with standardization of marketed herbal formulation Mustakarishta based on various parameters, TLC and HPTLC fingerprinting profiles. Mustakarishta was also prepared by in-house fermentation method as per procedure given in authorized books of Ayurveda following GMP guidelines. Raw herbal material was authenticated, standardized and used for preparation of arishta. It was found that results for all parameters that are tested were within acceptance criteria. Marker compound that isolated from raw plant mustaka and marketed Mustakarishta was characterized using spectral techniques. Both products were studied using fingerprint techniques by TLC and HPTLC in toulene: ethyl acetate (9:1 v/v) as mobile phase. Both techniques were found to be simple, rapid, reliable and economic.
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